Novel polymer system for molecular imprinting polymer against amino acid derivatives

Molecular imprinting polymers (MIPs) against N-Cbz-L-Tyr were prepared util izing different polymer systems and evaluated in HPLC mode. It was found th at MIP utilizing cocktail functional monomers, acrylamide + 2-vinylpyridine showed better molecular recognition and better separation ability for the template molecule than those utilizing other functional monomers. MIP utili zing trimethylolpropane trimethacrylate as cross-linker showed higher load capacity and separation factor than those utilizing ethylene glycol dimetha crylate as cross-linker. Increasing the concentration of competing solvent, acetic acid weakened the ionic inter-action and hydrogen bonding between t he analyte and the functional monomers, 2-vinylpyridine and acrylamide, whe n the template enantiomer was separated by HPLC. Therefore increasing of th e concentration of acetic acid leads to decreasing of capacity factor, sepa ration factor and resolution.