Properties and synthesis of new supports for immobilization of enzymes by copolymerization of vinylene carbonate and methacrylic acid

Methacrylic acid first was neutralized with an aqueous solution of NaOH to pH = 6.0 similar to 7.0, vinylene carbonate (VCA) was added to the solution , then monomers were copolymerized in paraffin oil by means of reverse-phas e suspension polymerization and hydrophilic copolymeric supports were prepa red. The properties of the supports were determined using trypsin and resul ts show that the amount of enzymes coupled to the supports and the specific activity of immobilized trypsin are related to the content of VCA structur e units, reaction time and concentration of enzyme solution, etc.