Gm. Beck et Sh. Neau, Optimization of lambda-carrageenan as a chiral selector in capillary electrophoresis separations, CHIRALITY, 12(8), 2000, pp. 614-620
Lambda-carrageenan, a linear high molecular weight sulfated polysaccharide,
was employed as a chiral selector in capillary electrophoresis for the sep
aration of enantiomers of weakly basic pharmaceutical compounds. In order t
o improve the utility of the chiral selector, the purity and concentration
of the lambda-carrageenan and other important capillary electrophoresis met
hod parameters were investigated. The results indicated that the purity and
concentration of the lambda-carrageenan, ionic strength of the buffer, and
temperature were critical to successful enantioseparation. These new metho
d conditions were then applied to previously investigated beta-blockers (su
ch as propranolol HCl and pindolol) and racemic tryptophan derivatives. The
se studies were successful in identifying important method conditions for t
he improved enantioselectivity with lambda-carrageenan. (C) 2000 Wiley-Liss
, Inc.