IGF-II and IL-2 act synergistically to alter HDAC1 expression following treatments with trichostatin A

Histone deacetylases play key roles in the regulation of gene transcription . Studies have shown that expression of interleukins IL-2 and IL-8, and ins ulin-like growth factor 2 (IGF2) are affected by treatment with histone dea cetylase inhibitors. We have previously shown that the gene for histone dea cetylase 1 (HDAC1) is upregulated following treatment with TSA, The murine homologue of this gene has been reported to be inducible by IL-2, In this s tudy, we have examined the effects IL-2, IGF-II and TSA have on HDAC1 expre ssion in the human hepatocellular carcinoma derived cell line Hep3B. Our re sults indicate that in contrast to the mouse, HDAC1 is not inducible by IL- 2, However, in TSA treated cells, IL-2 and IC;PII sere found to act synergi stically to reduce TSA induced HDAC1 mRNA levels almost to normal. (C) 2000 Academic Press.