IL-6 promoter is modulated by the 24 kDda FGF-2 isoform fused to the hormone binding domain of the oestrogen receptor

Authors
Delrieu, I Chinestra, P Delassus, F Bayard, F Prats, H Faye, JC
Citation
I. Delrieu et al., IL-6 promoter is modulated by the 24 kDda FGF-2 isoform fused to the hormone binding domain of the oestrogen receptor, CYTOKINE, 12(7), 2000, pp. 1110-1114
Citations number
15
Categorie Soggetti
Cell & Developmental Biology
Journal title
CYTOKINE
ISSN journal
10434666 → ACNP
Volume
12
Issue
7
Year of publication
2000
Pages
1110 - 1114
Database
ISI
SICI code
1043-4666(200007)12:7<1110:IPIMBT>2.0.ZU;2-9
Abstract
Fusion proteins consisting of the 24 kDa nuclear form of basic fibroblast g rowth factor (FGF-2), associated with the hormone binding domain of oestrog en receptor (HBD), convey oestrogen inducibility to FGF-2 When stable HBD-F GF-2 HeLa cell lines were transiently transfected with an interleukin 6 (IL -6) construct, the IL-6 promoter activity was downregulated by the addition of oestradiol. Moreover, in these cell lines, the function of the FGF-2 nu clear localisation sequence was abolished by its fusion to HBD, while addit ion of oestradiol restored the location of the chimera to the nucleus. (C) 2000 Academic Press.