Plasma and bile antibodies of the teleost Trematomus bernacchii specific for the nematode Pseudoterranova decipiens

Citation
Mr. Coscia et U. Oreste, Plasma and bile antibodies of the teleost Trematomus bernacchii specific for the nematode Pseudoterranova decipiens, DIS AQU ORG, 41(1), 2000, pp. 37-42
Citations number
23
Categorie Soggetti
Aquatic Sciences
Journal title
DISEASES OF AQUATIC ORGANISMS
ISSN journal
01775103 → ACNP
Volume
41
Issue
1
Year of publication
2000
Pages
37 - 42
Database
ISI
SICI code
0177-5103(20000525)41:1<37:PABAOT>2.0.ZU;2-L
Abstract
We investigated the occurrence of antibodies against protein antigens of th e nematode parasite Pseudoterranova decipiens in the plasma and bile of the Antarctic teleost Trematomus bernacchii. Three different P. decipiens prot ein solutions were prepared: excreted/secreted proteins from live larvae (E SP); surface-associated proteins obtained by mild extraction of larval bodi es (SAP); and cuticular soluble proteins recovered by extraction in strong reducing conditions (CSP). Using different immunoassays, these 3 preparatio ns were tested for their ability to bind fish antibody. As determined by EL ISA, the specific antibody binding activity was higher in SAP than in CSP. As determined by dot-blot immunoassay, the specific antigen binding activit y versus SAP was higher in bile than in plasma antibodies. A different numb er of antigenic components of SAP and ESP were identified by immunoblotting performed with plasma or bile antibodies. These results led to the conclus ion that T. bernacchii parasitism by nematodes involves plasma and bile ant i-parasite antibodies. Furthermore bile antibodies were found to be more re active and more heterogeneous than plasma.