A stability-indicating HPLC method to determine cyproterone acetate in tablet formulations

A simple and accurate liquid chromatographic method was developed to estima te cyproterone acetate (CA) in pharmaceuticals. The drug was chromatographe d on a reversed-phase C18 column. Eluents were monitored at a wavelength of 254 nm utilizing a mixture (60:40) of acetonitrile and water. Solution con centrations were measured on a weight basis to avoid the use of an internal standard. The method was statistically validated for linearity, accuracy, precision, and selectivity. Due to its simplicity and accuracy, we believe that the method can be used for routine quality control analysis. No specif ic sample preparation is required except for the use of a column guard and a suitable prefilter attached to the syringe.