Citation
L. Doretti et al., Acetylcholine biosensor involving entrapment of acetylcholinesterase and poly(ethylene glycol)-modified choline oxidase in a poly(vinyl alcohol) cryogel membrane, ENZYME MICR, 27(3-5), 2000, pp. 279-285
Citations number
21
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
ENZYME AND MICROBIAL TECHNOLOGY
ISSN journal
01410229
→ ACNP
Volume
27
Issue
3-5
Year of publication
2000
Pages
279 - 285
Database
ISI
SICI code
0141-0229(200008)27:3-5<279:ABIEOA>2.0.ZU;2-R
Abstract
A bienzymatic sensor for the determination of acetylcholine was prepared by
physical coimmobilization of acetylcholinesterase and poly(ethylene glycol
)-modified choline oxidase in a poly(vinyl alcohol) cryogel membrane obtain
ed by a cyclic freezing-thawing process. The enzyme-modified polymer was ap
plied on a platinum electrode to form an amperometric sensor, based on the
electrochemical detection of enzymatically developed hydrogen peroxide. The
analytical characteristics of this sensor, including calibration curves fo
r choline and acetylcholine, pH, and temperature effects, and stability an
described. (C) 2000 Elsevier Science Inc. All rights reserved.