The morphological development of neurons derived from EGF- and FGF-2-driven human CNS precursors depends on their site of integration in the neonatalrat brain
Ae. Rosser et al., The morphological development of neurons derived from EGF- and FGF-2-driven human CNS precursors depends on their site of integration in the neonatalrat brain, EUR J NEURO, 12(7), 2000, pp. 2405-2413
Neural precursor cells derived from the developing human brain were expande
d in vitro under the influence of fibroblast growth factor-2 (FGF-2) and ep
idermal growth hormone (EGF), and were then transplanted into different reg
ions of the neonatal rat brain. Four weeks later neurons were seen to have
developed from human embryonic precursors, using a human-specific antibody
to tau (htau). There were morphological differences between implanted neuro
ns developing in the hippocampus, striatum and neocortex, which were confir
med by cell volume measurements, although no specific neurochemical phenoty
pes were identified. Htau-positive fibres were seen to project extensively
along fibre pathways appropriate for the site of neuronal integration. This
study demonstrates that, following cell division in vitro, neurons differe
ntiating from human precursor cell populations retain the ability to respon
d appropriately to regional determinants present in the neonatal rat brain.
This is important for the application of such cells in CNS repair strategi
es, in particular neural transplantation.