Semi-selective medium for isolation of Colletotrichum gloeosporioides fromsoil

Isolation of Colletotrichum gloeosporioides (causal agent of yam anthracnos e) from soil is difficult because of the presence of fast growing micro-org anisms like Rhizoctonia spp., Rhizopus spp., Penicillium spp. and bacteria. Therefore, a semi-selective medium consisting of antimicrobial agents such as pencycuron 50 mg L-1, tolclofos-methyl 10 mg L-1, streptomycin sulphate 100 mg L-1, chloramphenicol 100 mg L-1 and chlortetracycline 100 mg L-1 wa s developed using potato dextrose agar as the basal medium. The procedure i nvolved screening of candidate fungicides, developing a suitable combinatio n of antimicrobial agents, assessing the proposed semi-selective medium and adjustment of pH of the medium to 5. The effectiveness of the medium is du e to selective inhibition of micro-organisms by the antimicrobial agents an d the development of distinct salmon-pink colonies of C. gloeosporioides.