Hypobiosis induction alters the protein profile of Ostertagia ostertagi (Nematoda : Trichostrongylidae)

Citation
Mc. Dopchiz et al., Hypobiosis induction alters the protein profile of Ostertagia ostertagi (Nematoda : Trichostrongylidae), FOL PARASIT, 47(2), 2000, pp. 135-140
Citations number
30
Categorie Soggetti
Biology
Journal title
FOLIA PARASITOLOGICA
ISSN journal
00155683 → ACNP
Volume
47
Issue
2
Year of publication
2000
Pages
135 - 140
Database
ISI
SICI code
0015-5683(2000)47:2<135:HIATPP>2.0.ZU;2-S
Abstract
The appearance of variations in the protein profile of Ostertagia ostertagi (Stiles, 1892) infective larvae (L-3), which were induced by hypobiosis tr iggering factors, was evaluated by means of SDS-PAGE and densitometric anal ysis. Area integration analyses of their protein profiles was carried out b etween 66 and 77 kDa. Important quantitative variations were identified in the protein levels of the induced larvae, where a 5.25 fold increase compar ed to the control was observed. Two 75.4 and 70 kDa protein bands were foun d which exceeded the control profile by 4.5 and 44 Cold, respectively. This fact suggests that the changes brought about in the process of hypobiosis induction are restricted. This work demonstrates changes at a molecular lev el corresponding with biological changes induced by conditions causing O. o stertagi hypobiosis.