Mc. Dopchiz et al., Hypobiosis induction alters the protein profile of Ostertagia ostertagi (Nematoda : Trichostrongylidae), FOL PARASIT, 47(2), 2000, pp. 135-140
The appearance of variations in the protein profile of Ostertagia ostertagi
(Stiles, 1892) infective larvae (L-3), which were induced by hypobiosis tr
iggering factors, was evaluated by means of SDS-PAGE and densitometric anal
ysis. Area integration analyses of their protein profiles was carried out b
etween 66 and 77 kDa. Important quantitative variations were identified in
the protein levels of the induced larvae, where a 5.25 fold increase compar
ed to the control was observed. Two 75.4 and 70 kDa protein bands were foun
d which exceeded the control profile by 4.5 and 44 Cold, respectively. This
fact suggests that the changes brought about in the process of hypobiosis
induction are restricted. This work demonstrates changes at a molecular lev
el corresponding with biological changes induced by conditions causing O. o
stertagi hypobiosis.