In this paper, we report the chromosomal localization of ceratotoxins, a ge
ne family encoding antibacterial female-specific peptides from the mediterr
anean fruit fly Ceratitis capitata. The analysis of both polytene and mitot
ic chromosomes by in situ hybridization shows that ceratotoxins are the fir
st case of female-specific X-linked genes from the medfly C. capitata. Sout
hern blot analysis reveals that the ceratotoxin gene family is not specific
ally amplified in the female reproductive accessory glands of C. capitata.