Characterization of wheat-Thinopyrum partial amphiploids by meiotic analysis and genomic in situ hybridization

A combination of genomic in situ hybridization (GISH) and meiotic pairing a nalysis of wheat-Thinopyrum partial amphiploids was employed to identify th e genomic constitution and relationships between partial amphiploids derive d from wheat and wheatgrass crosses. On the basis of similarities in the me iotic behavior and GISH patterns, the alien chromosomes of two of eight par tial amphiploids, TAF46 and 'Otrastayuskaya 38', were judged to originate f rom Th. intermedium, whereas Th. ponticum was one of the parents of the oth er six partial amphiploids; PWM706, PWM206, PWM209, PWMIII, OK7211542, and Ag-wheat hybrid. Each of these partial amphiploids was found to contain a s ynthetic alien genome composed of different combinations of St-, J-, or J(s )-genome chromosomes. For relatedness of partial amphiploid lines, meiotic analysis of F-1 hybrids and GISH results were generally complementary, but the latter offered greater precision in identifying constituent genomes.