Histological and genetic analysis and risk assessment for chromosomal aberration after ICSI for patients presenting with CBAVD

Intracytoplasmic sperm injection (ICSI) has opened a new field in the treat ment of male infertility, leading to a debate concerning its genetic safety , In this study we present an analysis of 11 patients presenting congenital bilateral absence of the vas deferens (CBAVD), In all 11 cases, genetic co unselling, histological analysis of testicular biopsies, cystic fibrosis tr ansmembrane conductance regulator (CFTR) mutation screenings of both partne rs and spermatozoa three-colour fluorescent in-situ hybridization (FISH) an alysis were performed. A total of 31 CFTR mutations were screened and mutat ions were found in eight out of 11 cases, with Delta F508 being the most co mmon mutation found. Histological analyses showed that seven out of 11 pati ents had normal tubule/membrane/interstitium (TMI) and Johnsen scores, whil e the remaining four patients had mild impairment of testicular parenchyma. The average aneuploidy rate was 6.8 +/- 3.9% compared with two control sub jects with 4.4 and 5.4% aneuploidy rates respectively, using FISH analysis. After ICSI, the fertilization and pregnancy rates were 66.2 and 22.7% resp ectively. Thus, in our case of CBAVD, the risk of chromosomal aberration fo llowing ICSI, in the absence of a CFTR mutation in the male patient and/or in his partner, was not higher than in normal fertile men. Furthermore, the pregnancy success rate following ICSI of these CBAVD patients was comparab le to the general ICSI population, even when histological analysis showed l imited spermatogenesis.