The effects of mutated skeletal ryanodine receptors on hypothalamic-pituitary-adrenal axis function in boars

The objectives of the current experiment were to determine whether boars he terozygous for the mutation in skeletal ryanodine receptors (sRyR), known t o cause porcine stress syndrome, differed from wild-type boars in hypothala mic-pituitary-adrenal axis (HPA) function. We have examined basal plasma AC TH, cortisol, and corticosteroid-binding globulin (CBC;) concentrations; pl asma ACTH and cortisol responses to a nose-snare stressor and at slaughter; dexamethasone suppression of plasma ACTH and cortisol concentrations; and glucocorticoid receptor (Gn) density in the pituitary gland, hippocampus, h ypothalamus, and frontal cortex. We have also examined carcass yields, comp osition, and meat quality to determine whether differences in HPA activity were accompanied by an increased incidence of meat quality characteristics associated with pale, soft, exudative (PSE) meat. Thirty boars either heter ozygous or wild-type (n = 15 per genotype) for mutated sRyR were tested for HPA function at 7 mo of age. Heterozygous boars had lower basal plasma ACT H (P < .05) and cortisol (P < .04) concentrations. Integrated basal plasma ACTH and cortisol levels were also lower (P <.05 and P < .005, respectively ). Genotype had no significant effect on basal CBG, stressor-induced (nose snare or slaughter) or dexamethasone suppression of plasma ACTH or cortisol concentrations. No differences in immunoreactive GR levels were found in t he pituitary gland or any brain region examined. We did find a significant, negative correlation (r = -.62, P < .02) between peak (0800) basal plasma ACTH concentrations and hippocampal GR levels. The alterations in basal HPA function in heterozygous boars were accompanied by lighter body weights (P < .03), decreased carcass fat depth (P < .04), and increased carcass lean yields (P < .02). There was a higher incidence of meat quality characterist ics associated with PSE meat in heterozygous boars indicated by higher carc ass temperatures (P < .04) and meat brightness (P < .0001) with lower carca ss pH at slaughter (P < .03) and after chilling (P < .003). In conclusion, we have found differences in basal and not stressor-induced HPA function be tween boars heterozygous and wild-type for mutated sRyR. This altered basal HPA activity was accompanied by an increased incidence of meat quality asp ects associated with PSE meat in heterozygous boars.