Cloning and characterization of G+C-rich, highly repeated DNA sequences from Porphyra yezoensis (laver), Rhodophyta

Citation
Y. Mizukami et al., Cloning and characterization of G+C-rich, highly repeated DNA sequences from Porphyra yezoensis (laver), Rhodophyta, J APPL PHYC, 12(2), 2000, pp. 131-138
Citations number
22
Categorie Soggetti
Aquatic Sciences
Journal title
JOURNAL OF APPLIED PHYCOLOGY
ISSN journal
09218971 → ACNP
Volume
12
Issue
2
Year of publication
2000
Pages
131 - 138
Database
ISI
SICI code
0921-8971(2000)12:2<131:CACOGH>2.0.ZU;2-O
Abstract
G+C-rich sequences in the genomic DNA of Porphyra yezoensis (laver) were cl oned and characterized. Sequence analyses of the genomic DNA inserted in fi ve clones showed that the DNA contained long G+C-rich stretches of more tha n 200 bp. These stretches consisted of more than 80% G+C residues. The G+C- rich sequences were highly repeated and interspersed throughout the genome of P. yezoensis and constituted about 6.0-6.6% of the genome. Parts of thes e sequences were tandem repeated in arrays. Hybridization experiments showe d that these highly repeated, interspersed G+C-rich sequences were present in other species of Porphyra, but not in species of the genera Grateloupia and Gelidium, suggesting that these sequences might evolve rapidly among ge nomes, species and genera.