Y. Mizukami et al., Cloning and characterization of G+C-rich, highly repeated DNA sequences from Porphyra yezoensis (laver), Rhodophyta, J APPL PHYC, 12(2), 2000, pp. 131-138
G+C-rich sequences in the genomic DNA of Porphyra yezoensis (laver) were cl
oned and characterized. Sequence analyses of the genomic DNA inserted in fi
ve clones showed that the DNA contained long G+C-rich stretches of more tha
n 200 bp. These stretches consisted of more than 80% G+C residues. The G+C-
rich sequences were highly repeated and interspersed throughout the genome
of P. yezoensis and constituted about 6.0-6.6% of the genome. Parts of thes
e sequences were tandem repeated in arrays. Hybridization experiments showe
d that these highly repeated, interspersed G+C-rich sequences were present
in other species of Porphyra, but not in species of the genera Grateloupia
and Gelidium, suggesting that these sequences might evolve rapidly among ge
nomes, species and genera.