Adsorption/desorption of protein on magnetic particles covered by thermosensitive polymers

The adsorption and desorption behavior of protein human serum albumin (HSA) on magnetic polymer particles covered by thermosensitive polymers was inve stigated. The results showed that adsorption was dependent mainly on the pr operties of the particle surface. By increasing the temperature, particles deswelled and were susceptible to absorb larger amounts of proteins, which could be desorbed at lower temperature. The extent of adsorption was found to depend on the pH value, protein concentration, and incubation time. At h igher pH, a smaller amount of proteins could be absorbed because of the ele ctrostatic repulsive force between the protein and the surface particles. I ncreasing incubation time or initial protein concentration was favorable to the adsorption of proteins. (C) 2000 John Wiley & Sons, Inc.