Ms. Dardanelli et al., Synthesis, accumulation and hydrolysis of trehalose during growth of peanut rhizobia in hyperosmotic media, J BASIC MIC, 40(3), 2000, pp. 149-156
We examined and compared the activities of synthetic and hydrolytic enzymes
involved in trehalose metabolism, in three peanut rhizobia strains grown i
n control, hypersaline, and non-ionic hyperosmotic media. Results indicated
that the effects of hyperosmolarity on the synthesis and the degradation o
f the disaccharide were diverse. In the salt-tolerant slow-growing strain B
radyrhizobium sp. ATCC 10317, we observed increased synthesis and accumulat
ion of trehalose under hyperosmolarity imposed by either NaCl or PEG-8000.
In the other two peanut rhizobia strains, the disaccharide level did not ch
ange under hypersalinity. In the salt-sensitive slow-growing strain Bradyrh
izobium sp. USDA 3187, intracellular trehalose diminished in late stationar
y phase-cells grown with PEG, this reduction was accompanied by both an inc
reased activity of synthetic enzymes and a decreased activity of trehalase.
In the salt-tolerant fast-growing strain Rhizobium sp. TAL. 1000, we also
observed a reduction of intracellular trehalose under PEG-mediated growth,
this decrease was early and transiently accompanied by an enhancement of tr
ehalase activity, afterwards, the activity of synthetic enzymes augmented.