Separation of gallic acid from Cornus officinalis Sieb. et Zucc by high-speed counter-current chromatography

Gallic acid was separated from a n-butanol extract of the fruit of Cornus o fficinalis Sieb. et Zucc by high-speed countercurrent chromatography in two steps using two solvent systems composed of ethyl-acetate-ethanol n-butano l-water (5:1.8:6, v/v/v) and ethyl acetate-ethanol-water (5:0.5:6, v/v/v) s uccessively. From 1 g of n-butanol extract the method produced 60 mg of gal lic acid at a purity of 97%. (C) 2000 Elsevier Science B.V. All rights rese rved.