Jc. Chalchat et al., Photochemical hydroperoxidation of terpenes. Antimicrobial activity of alpha-pinene, beta-pinene and limonene hydroperoxides, J ESSEN OIL, 12(1), 2000, pp. 125-134
The rose Bengal photosensitized oxidation of the (+) and (-) stereoisomers
of alpha-pinene, beta-pinene and limonene has been optimized to produce suf
ficient quantities of hydroperoxides for antimicrobial screening. Experimen
tal conditions allowed the production of highly concentrated solutions of h
ydroperoxides (approximately 2 M, approximately 30 w%). The antimicrobial a
ctivity of each hydroperoxide solution was evaluated by measuring their min
imum inhibitory concentration (MIC) (1,2). All the hydroperoxides behave as
powerful antimicrobial agents (similar to hydrogen peroxide). The MIC valu
es were almost independent of the nature of the parent terpene but depended
of the nature of the bacterial colony (five conventional families, met in
human pathologies, were tested).