Comparison of the free radical-scavenging activity of propolis from several regions of Argentina

Propolis is extensively used in Argentine folk medicine. Alcoholic extracts of propolis from different regions of Argentina were prepared. The extract s were analysed for the determination of total flavonoid content (from 13.3 to 42.6 mg/g of propolis) by using the aluminum nitrate method, UV spectro photometry and thin layer chromatography. All of them contained high total flavonoid content. It was also observed that all samples of ethanolic extra cts of propolis showed free radical-scavenging activity in terms of scaveng ing of the radical DPPH but the highest activities were found for samples f rom Tucuman and Santiago del Estero. In all cases with 20 mu g/ml of solubl e principles, the percentage of DPPH degradation was different (Banda Oeste : 67.5%; Veronica: 45%: Forres: 35%; Saenz Pena: 20% and Juan Jose Castelli : 55%). These results may justify their use as a source of natural antioxid ants. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.