Tecoma sambucifolia: anti-inflammatory and antinociceptive activities, and'in vitro' toxicity of extracts of the 'huarumo' of Peruvian Incas

Aqueous and alcoholic extracts of pods and flowers of Tecoma sambucifolia H .B.K. (Bignoniaceae) ('huarumo') were analysed to determine their anti-infl ammatory activity (carrageenan-induced edema test), antinociceptive activit y (acetic acid writhing test) and 'in vitro' toxicity in Chinese hamster ov ary cells, human hepatome cells and human larynx epidermal carcinoma cells. The cytotoxic effects of both extracts were evaluated by two endpoint syst ems: neutral red uptake assay and tetrazolium assay. The results showed tha t all extracts have anti-inflammatory and antinociceptive activity, but the highest potency is that of the alcoholic extracts. There were significant differences in cytotoxicity between extracts and among the response of cell s to them. The highest cytotoxicity was noted with the alcoholic extract, a nd the human hepatome cell :line was the most sensitive, especially to the alcoholic extract of flowers. The aqueous pod extract appeared to have the best pharmaco-toxicological profile, since it provided a significant reduct ion of both pain and inflammation together with the lowest cytotoxicity. (C ) 2000 Elsevier Science Ireland Ltd. All rights reserved.