Single polyprenol and dolichol isolation by semipreparative high-performance liquid chromatography technique

A new method of separation of single polyprenols (or dolichols) from a mixt ure of isoprenoid alcohols is described. Application of a high-performance liquid chromatography (HPLC) apparatus equipped with a semipreparative ODS column resulted in preparation of long-chain (dihydro)polyprenols of high p urity (>95%). This approach substantially decreases the time scale of the c onventional chromatographical preparative procedure. The method can be wide ly used in chemical and biochemical projects, where single polyprenols or d olichols are required.