Layer 1 of the developing rodent somatosensory cortex contains a dense, tra
nsient GABAergic fiber plexus. Axons arising from the zona incerta (ZI) of
the ventral thalamus contribute to this plexus, as do axons of intrinsic GA
BAergic cells of layer 1. The function of this early-appearing fiber plexus
is not known, but these fibers are positioned to contact the apical dendri
tes of most postmigratory neurons. Here we show that electrical stimulation
of layer 1 results in a GABA(A)-mediated postsynaptic current (PSC) in pyr
amidal neurons. Gramicidin perforated patch recording demonstrates that the
GABAergic layer 1 synapse is excitatory and can trigger action potentials
in cortical neurons. In contrast to electrical stimulation, activation of i
ntrinsic layer 1 neurons with a glutamate agonist fails to produce PSCs in
pyramidal cells. In addition, responses can be evoked by stimulation of lay
er 1 at relatively large distances from the recording site. These findings
are consistent with a contribution of the widely projecting incertocortical
pathway, the only described GABAergic projection to neonatal cortex. Recor
ding of identified neonatal incertocortical neurons reveals a population of
active cells that exhibit high frequencies of spontaneous action potential
s and are capable of robustly activating neonatal cortical neurons. Because
the fiber plexus is confined to layer 1, this pathway provides a spatially
restricted excitatory GABAergic innervation of the distal apical dendrites
of pyramidal neurons during the peak period of cortical synaptogenesis.