Dietary olive oil enhances murine lymphocyte calcium uptake

Citation
Md. Peck et al., Dietary olive oil enhances murine lymphocyte calcium uptake, J TRAUMA, 49(1), 2000, pp. 109-114
Citations number
50
Categorie Soggetti
Aneshtesia & Intensive Care
Volume
49
Issue
1
Year of publication
2000
Pages
109 - 114
Database
ISI
SICI code
Abstract
Background: Dietary lipids enhance immune function and improve outcome from injury or infection in animal models. We tested the hypothesis that amount , type, or both, of dietary lipid increases intracellular calcium concentra tion, a surrogate for lymphocyte activation. Methods: Mice were fed 2 weeks on semipurified diets with 5% (by weight [w/ w]), 10% (w/w), or 20% (w/w) dietary fat consisting of coconut, olive, saff lower, or linseed oil. Changes in intracellular calcium concentration after mitogen stimulation of splenic lymphocytes was estimated by using now cyto metry. Results: olive oil diets increase intracellular calcium concentration after concanavalin A, lipopolysaccharide, and CD3 stimulation. On the other hand , linseed oil (which is high in omega-3 fatty acids, which have been shown in other studies to enhance immune function) depresses intracellular calciu m levels. The amount of dietary fat had no effect on intracellular calcium. Conclusion:Olive oil merits further study in the application of nutritional pharmacology to immunomodulation of the critically injured, because it may enhance lymphocyte function.