The study examines an Er:YAG laser (2940 nm) and different application syst
ems of the CO2 laser (10 600 nm) with regard to their suitability for a one
-shot laser myringotomy of an adequate perforation size (similar to 2 mm).
The laser-tissue interaction of the Er:YAG laser and the CO2 laser in fresh
tympanic membranes of horses (thickness: 80-100 mu m) as well as in formal
in-fixed human tympanic membranes (thickness: 100 mu m) is studied correlat
ing perforation diameters to the applied power/energy density and the effec
ts demonstrated by light and scanning electron microscopy are analysed. Usi
ng the Er:YAG laser with a focused laser beam (spot diameter: 400 mu m) or
with a maximally defocused laser beam (spot diameter: 1600 mu m) perforatio
ns of an adequte size (2 mm) can only be achieved with multiple laser pulse
s. Histological studies disclose only minimal thermic side effects in the a
djacent tissue in both specimens. If the CO2 laser radiation is transmitted
via a silver halide polycrystalline fibre (diameter: 900 mu m) a maximal p
erforation diameter of 1300 mu m is achieved with significant thermic side
effects such as coagulation. Using an Acuspot(TM) 710 micromanipulator (foc
used beam diameter: 180 mu m) combined with a SilkTouch(TM) scanner a maxim
al perforation diameters of 1700 mu m can be achieved in horse tympanic mem
brane with one laser pulse. A prototype of a hand-held CO2 laser otoscope i
n combination with the SilkTouch(TM) scanner is suitable for performing las
er myringotomies with a diameter of 2 mm with a single laser pulse in fresh
horse tympanic membrane.