Effects of different wavelengths of low level laser irradiation on murine immunological activity and intracellular Ca2+ in human lymphocytes and cultured cortical neurogliocytes
M. Tong et al., Effects of different wavelengths of low level laser irradiation on murine immunological activity and intracellular Ca2+ in human lymphocytes and cultured cortical neurogliocytes, LASER MED S, 15(3), 2000, pp. 201-206
The purpose of this study was to determine the wavelength-response effects
of low level laser irradiation (LLLI) on immunocompetence of mice in vivo a
nd intracellular free calcium ([Ca2+](i)) in human lymphocytes and cultured
cortical neurogliocytes (CCN) in vitro. Mice were first immune compromised
by cyclophosphamide (CTX) injection, and the immunological activities incl
uding the production of interleukin 2 (IL-2), the murine mixed lymphocyte r
eaction (MLR), the mitogenic response of murine thymocytes (MRT), the proli
feration of murine bone marrow cells (BMC) and the natural killer (NK) cell
s activity, were investigated after intravascular low level laser irradiati
on (ILLLI) (1 mW, 1.1 x 10(4) J/cm(2)) at wavelengths of 532 nm, 632.8 nm,
650 nm and 1520 nm, respectively. In addition, using Ca2+ sensitive indicat
or Fura-2 AM with the Spex AR-CM-MIC cation measurement system ([Ca2+](i))
in single human lymphocytes and CCN were measured after LLLI (7.5 J/cm(2))
at wavelengths of 532 nm, 632.8 nm, 650 nm, 810 nm and 1300 nm, respectivel
y. Results showed that the ILLLI at wavelengths of 532 nm, 632.8 nm and 650
nm, produced a significant increase in the proliferation of BMC and the NK
activity. The production of IL-2 was greatly promoted after irradiation at
632.8 nm and 650 nm. After irradiation at 532 nm and 650 nm, the murine ML
R was evidently enhanced, and MRT was dramatically increased only after irr
adiation at 632.8 nm. In contrast, no significant effects were found on the
above mentioned indexes by irradiation at 1520 nm in comparison to the con
trol. In addition, [Ca2+](i) in single human lymphocytes and CCN were incre
ased after LLLI at wavelengths of 532 nm, 632.8 nm and 650 nm, respectively
, whereas they were not significantly affected by the wavelengths of 810 nm
and 1300 nm. Our results indicated that LLLI could induce significant and
different effects on the immunological activities of the mice and cause an
increase in [Ca2+](i) in single human lymphocytes and CCN. Furthermore, the
se effects are dependent on the wavelengths, for example, more positive eff
ects produced by the wavelengths of 532 nm, 632.8 nm and 652 nm than those
produced by the wavelengths of 810 nm, 1300 nm and 1520 nm.