T. Mio et al., Reduced virulence of Candida albicans mutants lacking the GNA1 gene encoding glucosamine-6-phosphate acetyltransferase, MICROBIO-UK, 146, 2000, pp. 1753-1758
The yeast GNA1 gene encodes glucosamine-6-phosphate acetyltransferase which
catalyses the reaction of glucosamine 6-phosphate with acetyl-CoA to form
N-acetylglucosamine 6-phosphate, a fundamental precursor in UDP-N-acetylglu
cosamine biosynthesis. Candida albicans mutants lacking GNA1 were viable in
the presence of N-acetylglucosamine. To confirm the physiological importan
ce of C. albicans GNA1, the virulence of a C. albicans gna1 Delta null muta
nt was examined in a mouse model of candidiasis. When injected intravenousl
y into mice, the virulence of the C. albicans gna1 Delta null mutant was si
gnificantly attenuated. The reduced virulence appeared to be the result of
rapid clearance from host tissue. These data suggest that C. albicans GNA1
is required for survival of the fungus in host animals, probably because an
insufficient level of N-acetylglucosamine is available from the host tissu
es.