Ed. Cambronne et al., LcrQ/YscM1, regulators of the Yersinia yop virulon, are injected into hostcells by a chaperone-dependent mechanism, MOL MICROB, 37(2), 2000, pp. 263-273
Pathogenic Yersinia species employ type III machines to secrete YopBDR into
the extracellular milieu. After attaching to host cells, yersiniae transfo
rm the type III machinery into an injection device and target YopEHMNOPT in
to eukaryotic cells. Yersinia pseudotuberculosis LcrQ is a transcriptional
regulator that prevents the expression of yop genes. We report that LcrQ is
injected into eukaryotic cells. YscM1, the transciptional regulator of Yer
sinia enterocolitica, is also injected into eukaryotic cells, whereas the r
elated YscM2 protein remains associated with bacterial cells. Type III targ
eting of YscM1 requires binding to the SycH chaperone. Chaperone binding as
well as depletion of YscM1 and YscM2 from the cytoplasm of Y. enterocoliti
ca causes an increase in yop expression, whereas a block in regulator expor
t reduces expression. We propose a model whereby the chaperone-mediated inj
ection of LcrQ/YscM1 functions as a regulatory switch for bacteria that are
attached to host cells, triggering the expression of Yops that travel the
type III targeting pathway.