Ea. Van Der Biezen et al., cDNA-AFLP display for the isolation of Peronospora parasitica genes expressed during infection in Arabidopsis thaliana, MOL PL MICR, 13(8), 2000, pp. 895-898
To identify genes from the obligatory biotrophic oomycete Peronospora paras
itica that are expressed during infection in Arabidopsis thaliana we employ
ed cDNA-amplified fragment length polymorphism (AFLP) display. cDNA-AFLP fr
agments from infected and non-infected leaves were separated in parallel by
gel electrophoresis and displayed by autoradiography, Most differential ge
ne fragments were derived from P. parasitica.