cDNA-AFLP display for the isolation of Peronospora parasitica genes expressed during infection in Arabidopsis thaliana

Citation
Ea. Van Der Biezen et al., cDNA-AFLP display for the isolation of Peronospora parasitica genes expressed during infection in Arabidopsis thaliana, MOL PL MICR, 13(8), 2000, pp. 895-898
Citations number
10
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
MOLECULAR PLANT-MICROBE INTERACTIONS
ISSN journal
08940282 → ACNP
Volume
13
Issue
8
Year of publication
2000
Pages
895 - 898
Database
ISI
SICI code
0894-0282(200008)13:8<895:CDFTIO>2.0.ZU;2-2
Abstract
To identify genes from the obligatory biotrophic oomycete Peronospora paras itica that are expressed during infection in Arabidopsis thaliana we employ ed cDNA-amplified fragment length polymorphism (AFLP) display. cDNA-AFLP fr agments from infected and non-infected leaves were separated in parallel by gel electrophoresis and displayed by autoradiography, Most differential ge ne fragments were derived from P. parasitica.