Time-resolved spectroscopy of the probe fluorescence in the study of humanblood protein dynamic structure on SR beam

Time-resolved spectroscopy on the SRS of the Daresbury Laboratory was used for the study of the human serum lipoproteins and human blood albumins with fluorescent probes K-37 and K-35, developed in Russia. The probe K-37 was found sensitive to the difference in dynamic properties of the lipid object s. Two sets of the parameters were used for the description of lipid dynami c structure: (1) time-resolved fluorescence spectra and (2) time-resolved f luorescence depolarization as a function of rotational mobility of lipid mo lecules. Each measured dynamic parameter reflected the monotonous changes o f dynamic properties in the range: lipid spheres-very low density lipoprote ins-low density lipoproteins-high density lipoproteins-phospholipid liposom es, The range is characterized by the increase of the ratio polar/nonpolar lipids. Thus, time-resolved fluorescence could be used to detect some struc tural modifications in lipoproteins related to atherosclerosis and subseque nt cardiovascular diseases development. (C) 2000 Elsevier Science B.V. All rights reserved.