Relationship between the ability to support differentiation of osteoclastlike cells and adipogenesis in murine stromal cells derived from bone marrow

In vitro osteoclast differentiation is supported by stromal cells. In order to isolate a stromal cell line that can support osteoclast differentiation , 22 cell lines were cloned from mouse bone marrow. One of these clones, TM S-14, is a line of preadipocytes that supports osteoclast-like cell formati on without any bone resorbing factors; and another, TMS-12, is a line of pr eosteoblasts that supports osteoclast-like cell formation with bone resorbi ng factors such as prostaglandin E-2 (PGE(2)). The difference of these two lines for osteoclast formation was not related with their abilities of PGE, production, but with the expression of osteoclast differentiation factor ( ODF, also called OPGL, RANKL, and TRANCE), which detected with RT-PCR, in b oth cell lines. In TMS-14 cells, ODF mRNA was detected with or without PGE, . In TMS-12 cells, ODF expression was detected in the PGE(2)-treated cells alone. When TMS-14 cells were induced to undergo adipogenic differentiation in response to treatment with thiazolidinedione, a ligand and activator of peroxisome proliferator-activated receptor gamma (PPAR gamma), the ability of TMS-14 cells to support osteoclast-like cell formation was prevented in the presence or absence of 1,25(OH)(2)D-3 The gene expression of ODF in TM S-14 cells was also inhibited by treatment with thiazolidinedione. These re sults suggest that adipogenesis in bone marrow cells is related to the abil ity to support osteoclast differentiation. This is the first report of a cl oned stromal cell line that can support osteoclastogenesis without the trea tment with any osteotropic factors. Furthermore, this murine clonal preadip ose cell line may be useful for studying senescence-dependent osteoporosis. (C) 2000 Harcourt Publishers Ltd.