Glucose biosensor enhanced by nanoparticles

Glucose biosensors have been formed with glucose oxidase (GOD) immobilized in composite immobilization membrane matrix, which is composed of hydrophob ic gold, or hydrophilic gold, or hydrophobic silica nanoparticles, or the c ombination of gold and silica nanoparticles, and polyvinyl butyral (PVB) by a set-gel method. The experiments show that nanoparticles can significantl y enhance the catalytic activity of the immobilization enzyme. The current response can be increased from tens of nanoamperometer (nA) to thousands of nanoamperometer to the same glucose concentration, and the electrodes resp ond very quickly, to about 1 min. The function of nanoparticles effect on i mmobilization enzyme has been discussed.