Kinetics of nitrogen uptake by Populus tremuloides in relation to atmospheric CO2 and soil nitrogen availability

Sustained increases in plant production in response to elevated atmospheric carbon dioxide (CO2) concentration may be constrained by the availability of soil nitrogen (N). However, it is possible that plants will respond to N limitation at elevated CO2 concentration by increasing the specific N upta ke capacity of their roots. To explore this possibility, we examined the ki netics of (NH4+)-N-15 and (NO3-)-N-15 uptake by excised roots of Populus tr emuloides Michx. grown in ambient and twice-ambient CO2 concentrations, and in soils of low- and high-N availability. Elevated CO2 concentration had n o effect on either NH4+ or NO3- uptake, whereas high-N availability decreas ed the capacity of roots to take up both NH4+ and NO3. The maximal rate of NH4+ uptake decreased from 12 to 8 mu mol g(-1) h(-1), and K-m increased fr om 49 to 162 mu mol l(-1), from low to high soil N availability Because NO3 - uptake exhibited mixed kinetics over the concentration range we used (10- 500 mu mol l(-1)), it was not possible to calculate V-max and K-m. Instead, we used an uptake rate of 100 mu mol g(-1) h(-1) as our metric of NO3- upt ake capacity, which averaged 0.45 and 0.23 mu mol g(-1) h(-1) at low- and h igh-N availability, respectively. The proximal mechanisms for decreased N u ptake capacity at high-N availability appeared to be an increase in fine-ro ot carbohydrate status and a decrease in fine-root N concentration. Both NH 4+ and NO3 uptake were inversely related to fine-root N concentration, and positively related to fine-root total nonstructural carbohydrate concentrat ion. We conclude that soil N availability, through its effects on fine-root N and carbohydrate status: has a much greater influence on the specific up take capacity of P. tremuloides fine roots than elevated atmospheric CO2. I n elevated atmospheric CO2, changes in N acquisition by P. tremuloides appe ared to be driven by changes in root architecture and biomass, rather than by changes in the amount or activity of N-uptake enzymes.