Rapid and direct sequencing of double-stranded DNA using exonuclease III and MALDI-TOF MS

Authors
Puapaiboon, U Jai-nhuknan, J Cowan, JA
Citation
U. Puapaiboon et al., Rapid and direct sequencing of double-stranded DNA using exonuclease III and MALDI-TOF MS, ANALYT CHEM, 72(14), 2000, pp. 3338-3341
Citations number
24
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ANALYTICAL CHEMISTRY
ISSN journal
00032700 → ACNP
Volume
72
Issue
14
Year of publication
2000
Pages
3338 - 3341
Database
ISI
SICI code
0003-2700(20000715)72:14<3338:RADSOD>2.0.ZU;2-G
Abstract
Application of MALDI-TOF MS to direct sequencing of dsDNA substrates is dem onstrated using a strategy that employs exonuclease III digestion of a targ et sequence. Experimental conditions for exonuclease III have been optimize d for this application, including addition of essential divalent metal ion cofactors. A short cation-exchange column was designed to provide efficient sample cleanup and overcome major problems arising from salt interference.