P. Ramesh et S. Sampath, A binderless, bulk modified, renewable surface amperometric sensor for NADH and ethanol, ANALYT CHEM, 72(14), 2000, pp. 3369-3373
Graphite particles are exfoliated and subsequently functionalized with tolu
idine blue. The resulting covalently modified graphite particles are restac
ked without any binder to form a surface-renewable, bulk-modified electrode
. Electrocatalytic oxidation of NADH and its application in the amperometri
c biosensing of ethanol using alcohol dehydrogenase enzyme have been demons
trated with this material.