Relationship between semen quality and performance of instrumentally inseminated honey bee queens

Techniques to effectively store honey bee semen must meet some minimally ac ceptable level of spermatozoa survival. To determine this level, honey bel queens were inseminated using various mixes of Fresh and freeze-killed seme n, and were allowed to lay eggs in small colonies for three weeks. The quee ns receiving all freeze-killed spermatozoa (0% fresh) had no spermatozoa in their spermathecae, and produced only drone pupae (unfertilized eggs). The proportions of live and dead spermatozoa (determined by dual fluorescent s taining) in the spermathecae of queens receiving 25 to 100% fresh semen wer e not significantly different at 27 days post-insemination. Queens receivin g 50% Fresh semen or more produced only worker pupae (all eggs were fertili zed). Therefore, a program to improve storage of semen should only have to reach survival levels of 50% of the spermatozoa to have functional semen.