Adsorption of microorganism components by lixelle beads

We reported previously that Lixelle, which was used for beta-2 microglobuli n (BMG) adsorption columns, could adsorb not only BMG but also inflammatory cytokines. We then were interested in the application of Lixelle to patien ts with systemic inflammatory response syndrome (SIRS) and tried to find ou t its ability to adsorb microorganism components in vitro using lipopolysac charide (LPS) (E. coli: B8), endotoxin (ET) containing water, and peptidogl ycan (PG: Micrococcus luteus). The initial concentrations of each solution were LPS (ET: 29,135 EU/L), contaminated water (ET: 3,523 EU/L), and PG (67 .1 ng/ml) and 2.5 ml of each of the stock solutions and adjusted diluted so lutions contained 0.5 ml of Lixelle beads. After shaking at 37 degrees C fo r 2 h, ET in the solutions was determined by the ET specific-limulus ameboc yte lysate (ES-LAL) method and PG by the silkworm larbae plasma (SLP) metho d. The results revealed that even when ET concentrations in LPS and contami nated water were high, the samples containing Lixelle beads showed signific ant decreases. There was some adsorption of PG but no significant differenc es. Thus, Lixelle beads can adsorb not only BMG but also microorganism comp onents such as ET and PG. These findings, together with the ability to adso rb inflammatory cytokines by Lixelle, show the possibility of application f or the treatment of infectious SIRS.