Glucose deprivation does not affect GLUT1 targeting in 3T3-L1 adipocytes

We have previously demonstrated that glucose deprivation alters the glycosy lation of the GLUT1 glucose transporter in 3T3-L1 adipocytes. Many aberrant ly glycosylated proteins are retained in the endoplasmic reticulum by inter action with chaperones. Herein, we use three independent procedures to show that GLUT1 is targeted to the plasma membrane, despite alterations in glyc osylation, While earlier experiments revealed that plasma membrane targetin g of aglyco GLUT 1 transporter was significantly reduced, our data show for the first time that altered glycosylation provides sufficient information to drive appropriate trafficking. (C) 2000 Academic Press.