Nucleotide sequence, genomic organization and cell-cycle-dependent expression of a Chlamydomonas 14-3-3 gene

Members of the 14-3-3 protein family have been identified as regulatory ele ments in intracellular signalling pathways and cell cycle control. Previous ly we reported the nucleotide sequence of a 14-3-3 cDNA cloned from the uni cellular green alga Chlamydomonas reinhardtii. In this communication, we de scribe the nucleotide sequence, the genomic organization and the cell-cycle -dependent expression of the corresponding gene. The coding sequence of thi s gene was found to be interrupted by four introns of 124, 116, 81, and 659 bp, respectively. Introns 2-4 were found in conserved positions as compare d to the Arabidopsis 14-3-3 genes. A counterpart to intron 1 absent in the Arabidopsis 14-3-3 genes was found in the human 14-3-3 epsilon gene. (C) 20 00 Elsevier Science B.V. All rights reserved.