Metalloporphyrin catalyzed oxidation of N-hydroxyguanidines: A biomimetic model for the H2O2-dependent activity of nitric oxide synthase

A chemical model for the H2O2 promoted oxidation by nitric oxide synthase ( NOS) has been developed. Biomimetic oxidations were carried out using H2O2 and tetrakis(perfluorophenyl)porphyrinato-iron(III) chloride (FeTPPF20) as a catalyst. Similarly to NOS our model system produces N-delta-cyanoornithi ne, citrulline and NO from NOHA and did not oxidize arginine itself. Based on these results we propose a peroxide shunt to be involved in the catalyti c cycle of NOS. To the best of our knowledge this is the first chemical sys tem that semiquantitatively mimics NOS activity. (C) 2000 Elsevier Science Ltd. All rights reserved.