L. Dominguez et al., Use of L4 larvae of Nippostrongylus brasiliensis for the in vivo screeningof anthelmintic drugs, CAN J VET R, 64(3), 2000, pp. 160-163
Citations number
20
Categorie Soggetti
Veterinary Medicine/Animal Health
Journal title
CANADIAN JOURNAL OF VETERINARY RESEARCH-REVUE CANADIENNE DE RECHERCHE VETERINAIRE
Five groups of Wistar rats were subcutaneously infected with 5000 L3 stage
larvae of Nippostrongylus brasiliensis. Four groups were dosed orally with
5, 10, 15, and 20 mg/kg body weight of albendazole at 5 h postinfection, an
d one group served as an untreated control. The animals were euthanized and
the intestines were dissected out 72 h postinfection and were incubated on
a Baermann's apparatus to recover L4 stage larvae. No larvae were recovere
d from the 20 mg/kg albendazole-treated group, and means of 15, 33, and 175
L4 larvae were recovered from the 15, 10, and 5 mg/kg albendazole-treated
groups, respectively. Levamisole and fenbendazole were also tested using th
e same procedure. Means of 100 and 1887 L4 larvae were obtained from the 50
and 10 mg/kg of levamisole-treated groups respectively; while, 191 and 583
L4 larvae were recovered from rats treated with 50 and 10 mg/kg of fenbend
azole, respectively. These results indicate that the L4 stage of N, brasili
ensis could be useful for in vivo screening of new nematocide drugs.