Experimental verifications on chemical carcinogenesis, a bifunctional alkylation between DNA interstrands

It is evidenced by the filter elution method that two carcinogenic aromatic hydrocarbons, benzo[a]pyrene and dibenzo[a,h]anthracene, two carcinogenic metal salts, beryllium chloride and cadmium chloride, four carcinogenic aro matic amines, 2-aminofluorene, beta-naphthylamine, 4-aminobiphenyl and benz idine, can all induce DNA interstrand and DNA-protein cross-link in L-1210 culture. However, under the same condition, the corresponding non-carcinoge nic compounds, including benzo[k]fluorancene, anthracene, magnesium chlorid e, zinc chloride, alpha-naphthylamine, 2-aminobiphenyl and m-toluidine, can not produce any cross-link adducts. Atl these results are consistent with t he di-region theory that carcinogens are bio-bifunctional alkylation agents . This method can also be used to discriminate carcinogens and non-carcinog ens.