Two-dimensional gel electrophoresis analysis of the proteomes expressed inthe human hepatoma cell line BEL-7404 and normal liver cell line L-02

Proteome analysis technology has been used extensively in conducting discov ery research of biology and has become one of the most essential technologi es in functional genomics. The proteomes of the human hepatoma cell line BE L-7404 and the normal human liver cell line L-02 have been separated by hig h resolution two-dimensional gel electrophoresis (2-DE) with immobilized pH gradient isoelectric focusing (IPG-IEF) in the first dimension and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in the second dimension (IPG-DALT). The resulting images have been analyzed using 2-D an alysis software. Quantitative analysis reveals that 7 protein spots are det ected only in hepatoma BEL-7404 cells, 14 only in L-02 cells, and 78 protei n spots show significant fluctuation in quantity in both cell lines (P<0.01 ). These protein spots have been displayed on a proteome differential expre ssion map. Analysis for the reproducibility of 2-DE indicates that the posi tional variability in the IEF dimension is 0.73 mm, while the variability i n the SDS-PAGE dimension is 0.44 mm, and the quantitative variability is 17 .6%-19.2%. These results suggest that the reproducibility of 2-DE has been, suitable for the study of differential expression of proteomes. Proteome d ifferential expression maps can be useful tools for disease diagnosis, drug -target validation analysis and biological process elucidation.