This article presents a sensitive electrochemical method for the quantifica
tion of proteins. The assay used the biuret reaction, which is based on the
complexation of proteins with copper ions to form a copper-protein complex
. In the present approach, once copper ions were complexed with the protein
, they were released by an acidic treatment and simultaneously separated by
ultracentrifugation from the protein sample. This allowed differential pul
se anodic stripping voltammetry (DPASV) of Cu2+ on carbon rotogravure print
ed electrodes (CARPEs). A nM detection limit for bovine serum albumin (BSA)
was found with this method. The kinetics of this assay were tested and it
resulted that the total assay can be carried out in 27 min, including incub
ation, washing steps and detection.