Electrochemical studies of the hemin-DNA interaction

An electrochemical study of the interactions of hemin with DNA on a hanging mercury drop electrode (in ammonia buffer solution) is described. Adding D NA in hemin solution resulted in decrease of both the hemin reduction peak and the catalytic hydrogen wave. Electrochemically nonactive hemin-DNA comp lexes are formed due to hemin intercalated into DNA. Then the equilibrium c oncentration of hemin in solution decreases. In this article, we give some support to the fact that hemin, as a dimer under our experiment, with a lar ge steric structure and anionic substitute on porphyrin plane, shows the ab ilities to intercalate into DNA.