Potential role of PKR in double-stranded RNA-induced macrophage activation

In this study, the role of the double-stranded (ds) RNA-dependent protein k inase (PKR) in macrophage activation was examined. dsRNA [polyinosinic:poly cytidylic acid (poly IC)]-stimulated inducible nitric oxide synthase, inter leukin (IL)-1 alpha and IL-1 beta mRNA expression, nitrite formation and IL -1 release are attenuated in RAW264.7 cells stably expressing dominant nega tive (dn) mutants of PKR. The transcriptional regulator nuclear factor (NF) -kappa B is activated by dsRNA, and appears to be required for dsRNA-induce d macrophage activation. While dnPKR mutants prevent macrophage activation, they fail to attenuate dsRNA-induced I kappa B degradation or NF-kappa B n uclear localization. The inhibitory actions of dnPKR on dsRNA-induced macro phage activation can be overcome by treatment with interferon (IPN)-gamma, an event associated with PKR degradation. Furthermore, dsRNA + IFN-gamma st imulate inducible nitric oxide synthase expression, I kappa B degradation a nd NF-kappa B nuclear localization to similar levels in macrophages isolate d from PKR-/- and PKR+/+ mice. These findings indicate that both NF-kappa B and PKR are required for dsRNA-induced macrophage activation; however, dsR NA-induced NF-kappa B activation occurs by PKR-independent mechanisms in ma crophages, In addition, the PKR dependence of dsRNA-induced macrophage acti vation can be overcome by IFN-gamma.