A variation of the amplified-fragment length polymorphism (AFLP) techniqueusing three restriction endonucleases, and assessment of the enzyme combination Bg/II-MfeI for AFLP analysis of Salmonella enterica subsp enterica isolates

We have performed amplified-fragment length polymorphism (AFLP) fingerprint ing on a collection of Salmonella enterica subsp. enterica serovar typhimur ium strains with a restriction endonuclease combination (BglII and MfeI) th at has previously been used successfully for typing Campylobacter jejuni is olates with high resolution. Additionally, a variation of the AFLP assay in which two rare cutting restriction enzymes (XbaI and BsrGI) in combination with the frequent cutter (HinP1I) was examined. The BglII and MfeI enzyme combination offered low resolution for genotyping Salmonella typhimurium is olates and is not recommended for this common serovar. The three-enzyme com bination gave a higher discrimination, and is thus a new alternate way of p erforming AFLP fingerprinting of S. typhimurium. (C) 2000 Federation of Eur opean Microbiological Societies. Published by Elsevier Science B.V. All rig hts reserved.