An enzyme linked immunosorbant assay (ELISA) for testosterone, estradiol, and 17,20 beta-dihydroxy-4-pregenen-3-one using acetylcholinesterase as tracer: application to measurement of diel patterns in rainbow trout (Oncorhynchus mykiss)

Authors
Nash, JP Cuisset, BD Bhattacharyya, S Suter, HC Le Menn, F Kime, DE
Citation
Jp. Nash et al., An enzyme linked immunosorbant assay (ELISA) for testosterone, estradiol, and 17,20 beta-dihydroxy-4-pregenen-3-one using acetylcholinesterase as tracer: application to measurement of diel patterns in rainbow trout (Oncorhynchus mykiss), FISH PHYS B, 22(4), 2000, pp. 355-363
Citations number
21
Categorie Soggetti
Aquatic Sciences
Journal title
FISH PHYSIOLOGY AND BIOCHEMISTRY
ISSN journal
09201742 → ACNP
Volume
22
Issue
4
Year of publication
2000
Pages
355 - 363
Database
ISI
SICI code
0920-1742(200005)22:4<355:AELIA(>2.0.ZU;2-B
Abstract
A simple and rapid Enzyme Linked ImmunoSorbant Assay (ELISA) is described a nd validated for testosterone, estradiol, and 17,20 beta-dihydroxy-4-pregne n-3-one (17,20 beta P). A general procedure for preparation of the acetylch olinesterase labeled steroid is described which is applicable to any steroi d. Use of acetylcholinesterase tracer increased the sensitivity of assay so that reliable measurements of each steroid could be achieved with only 10 mu l of plasma. The ELISA was applied to measurement of all three steroids every hour for over 24 hours in a female trout using cannulation of the dor sal aorta. This high sampling frequency revealed several short-term (< 2 h) episodic pulses of testosterone and estradiol.