The growth regulators used for bud regeneration and shoot rooting affect the competence for flowering and seed set in regenerated plants of protein peas
Sj. Ochatt et al., The growth regulators used for bud regeneration and shoot rooting affect the competence for flowering and seed set in regenerated plants of protein peas, IN VITRO-PL, 36(3), 2000, pp. 188-193
The production of whole plants from explants of protein pea (Pisum sativum
L.) using an efficient, reliable and rapid strategy, while maintaining true
ness to type, will be required before regeneration can be exploited for gen
etic transformation, Seeds of the pea genotypes Terese, Solara, Frisson and
P64 (a hypernodulating mutant line of Frisson) were surface-sterilized and
imbibed overnight, whereafter embryo axes were dissected and germinated on
hormone-free medium for 7-10 d. Hypocotyl sections lacking pre-existing me
ristems were harvested and cultured on a range of media with various concen
trations and combinations of growth regulators in order to induce either ca
ulogenesis or somatic embryogenesis. Differences in responsiveness were app
arent between genotypes, but regeneration via caulogenesis was consistently
more reliable than via the induction of somatic embryos. Few explants unde
rwent somatic embryo production and their conversion into plants has remain
ed elusive so far, irrespective of the genotype studied. Conversely, large
numbers of buds were produced within 10 d by organogenesis, and healthy, ro
otable shoots were obtained. A clear relationship was observed between the
growth regulators employed for bud regeneration and shoot routing phases an
d the subsequent competence of the regenerated plants for flowering, pod fo
rmation and viable seed production.